Dados do Trabalho

Título

PRESENCE OF ANTIMICROBIALS-RESISTANT BACTERIA IN WILD AND DOMESTIC ANIMALS IN PIAUÍ – BRAZIL

Introdução

The use, misuse and overuse of antimicrobials is one of the main public health threats of the 21st century, leading to the accelerated emergence of antimicrobial resistance (AMR) and consequent health and economical burdens. However, studies are still scarce, especially in the North and Northeast regions of Brazil.

Objetivo(s)

This study aimed to investigate the presence of antimicrobials-resistant bacteria in feces of wild birds and domestic animals in Piaui State. Brazil.

Material e Métodos

The sampled animals were distributed in five municipalities: Amarante, Água Branca, Lagoa Alegre, Parnaíba and Teresina. A convenience sampling was made, and anal or cloacal swabs of wild birds, chickens, equine, swine, and ruminants were taken. The swabs were kept in Amies charcoal medium at room temperature and sent to the Laboratory of Bacterial Resistance and Therapeutic Alternatives at the University of São Paulo (ICB-USP). The samples were screened using culture and antibiogram tests containing 16 antimicrobial-impregnated discs, which covered several antimicrobials classes used in the medical and veterinary routine: Additionally, a Maldi-TOF test were used to identify the bacteria.

Resultados e Conclusão

387 fecal samples were collected and 59 (15.2%) of these samples were identified as Extended Spectrum Beta-lactamases (ESBL). From all ESBL positive, 57 were identified as Escherichia coli and two as Klebsiella pneumoniae. Animals with the closest contact with humans showed the highest prevalence of positive ESBL: chickens (15.2%; n= 9/59), ruminants (19.5%; n= 16/82), and swine (39.4; n= 30/76) The largest cities in Piaui, Teresina and Parnaíba, had the highest prevalence of animals with ESBL resistant bacteria, with 32.0% (n=25/78) and 27.14% (n=19/70) respectively. Multiresistant microbiomes were observed in 72,9% of the samples (43/59). Among the positive samples for ESBL, 87,6% had resistance to the sulfonamides class, 85,5% to the tetracyclines class and 79,4% to the monobactams class.
Poor information and health care in Piaui, in addition to easy acquisition of antimicrobials without a prescription, favors the wrong ways of using antimicrobials in Piauí. Animals can be used as sentinels of rural and urban environmental health through the presence of AMR bacteria in their feces. E. coli and K. pneumoniae are species that can cause much harm to human health. More studies are necessary to elucidate the contamination of AMR in the environment.

Palavras-chave

Antibiotic, Escherichia coli, Klebsiella pneumoniae, Microbiology.

Área

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