Dados do Trabalho

Título

DIVERSITY OF TRYPANOSOMATIDS IN SOUTHERN COATIS (NASUA NASUA) INHABITING URBAN FORESTED FRAGMENTS IN CENTRAL WESTERN BRAZIL

Objetivo(s)

The present study aimed to access the trypanosomatid diversity of Southern coatis inhabiting forested fragments located in the urban area of Campo Grande municipality, Mato Grosso do Sul state, Brazil.

Material e Métodos

A total of 110 Southern coatis were captured between March 2018 and April 2019 in two forested fragments, (i) Prosa State Park (PEP), a Conservation Unit, and (ii) Air-Force Private Area (AFPA), a residential area. The animals were captured through Box traps, sedated using zolazepam hydrochloride and tiletamine hydrochloride and identified with subcutaneous microchips. Blood and bone marrow samples were collected from femoral vein and manubrium sterni respectively. The samples were submitted to phenol-chloroform DNA extraction and screened for trypanosomatid DNA detection through nested polymerase chain reaction (nPCR) using specific primers to the SSU rRNA region. The PCR products with best band quality and DNA concentration were pooled according to each forested fragment and submitted to Next Generation Sequencing (NGS) in Illumina HiSeq PE250 platform. The obtained sequences were analyzed through the DADA2 v1.140 package in the software R v3 6.2, using the SILVA v132 database, and Amplicon Sequence Variants (ASV) were constructed.

Resultados e Conclusão

A total of six blood samples from PSP and six bone marrow samples from AFPA were selected from the screening nPCR and submitted to NGS. Our analysis revealed that ASVs of Leishmania guyanensis, L. infantum, T. cruzi genotype TcI, T. lainsoni, T. rangeli and Trypanosoma sp. DID were found in both conserved and residential areas, while Neobodo designis, T. cruzi marinkellei and T. cruzi genotypes TcII were found only in PEP. Our results indicate that trypanosomatid richness of Southern coatis that inhabit forested urban fragments is higher in conserved than anthropized area, probably a consequence of habitat degradation and loss of biodiversity. Since the parasitism for L. infantum and L. guyanensis has already been reported in humans at Campo Grande, the role of Southern coatis in the leishmaniasis epidemiology in this area must be better understood

Palavras-chave

Key-words: Leishmania spp; Medium-sized carnivores; Trypanosoma spp.; Urban area