Dados do Trabalho

Título

DETECTION OF TRYPANOSOMATIDS SPECIES IN FREE-LIVING AND CAPTIVE CAPUCHIN MONKEYS (SAPAJUS CAY) SAMPLED IN CAMPO GRANDE, MATO GROSSO DO SUL

Objetivo(s)

The aimed evaluate the trypanosomatid richness in capuchin monkeys (Sapajus cay) captured in forest fragments and in the Wildlife Rehabilitation Center at Campo Grande (CG), Mato Grosso do Sul.

Material e Métodos

We used Box traps and baited with banana, papaya and eggs. The animals were sedated with 0.5 mg/kg midazolam and 12 mg/kg ketamine. Approximately 3 mL of blood was collected from via femoral vein puncture, deposited in clot activator tubes and kept under refrigeration at 4°C. We made hemoculture (HC) by inoculating 300 μL of blood in Novy McNeal Nicole (NNN) medium with liver infusion tryptose. Field procedures were in accordance with licenses granted by the Chico Mendes Instituto de Conservação da Biodiversidade (70946-3 / 2020) and approved by the Ethics Committee for the Use of Animals of the Catholic University Dom Bosco, Campo Grande (001/2020). We obtained 51 samples of S. cay, DNA was extracted from 50µL of clot samples by the ammonium acetate precipitation method. Molecular detection was performed by nested polymerase chain reaction (nPCR) using primers directed to 18S rRNA gene. We submitted 28 positives samples to the nPCR for Next Generation Sequencing (NGS) in Illumina HiSeq PE250 platform. The NGS-generated data were analyzed using DADA2(v1.14.0) in the R environment. Further, taxonomy of kinetoplastids were assigned using SILVA v132.

Resultados e Conclusão

The NGS revealed reads of Trypanosoma cruzi in 82.1% (23/28), five displayed single infection by TcI, three by TcII genotype, and TcI/TcII were observed in 15 animals. Leishmania infantum was detected in 78,5% and Leishmania guyanensis in 14,2%. Moreover, we found infection T. minasense in 46,4%, Trypanosoma sp. DID in all sampled capuchin monkey, and single infection by T. lainsoni and Bodo saltans. Regarding T. rangeli, we found reads in two animals, one of them with high parasitemias, as proved by positivity in HC (GenBank access number ON364108). Our results indicate that fragmentation of the landscape in urban environments may favor a change of the host specificity (host switch phenomenon) of some trypanosomatids species. Moreover, the role of capuchin monkeys in the transmission cycles of the multi-host parasites L. infantum and T. cruzi should be better known in the studied area.

Palavras-chave

Key-words: Wild host, Trypanosoma, Leishmania, Molecular detection, Brazilian Midwest