Dados do Trabalho

Título

Isolation of emerging yeasts from the blue-fronted parrot, Amazona aestiva

Introdução

The close relationship between humans and animals, the encroachment into wildlife areas, animal trafficking, and the presence of wild birds in urban and peri-urban environments contribute to the interspecies transmission of microorganisms. The keeping of wild animals from the passerine and psittacine groups is popular in Brazil, many of which are obtained through illegal trafficking and capture. Monitoring the microbiome associated with birds can indicate the circulation of specific etiological agents and is part of the necessary public health policies in the context of the One Health approach, for the management and mitigation of emerging infectious diseases.

Objetivo (s)

Considering the importance of monitoring the wildlife microbiota, this study aimed to identify yeasts from clinical samples collected from Amazona aestiva specimens from CETAS-IBAMA-BA, attended by the Wildlife and Exotic Animal Department of the Veterinary Medicine Hospital of the Federal University of Bahia.

Material e Métodos

The project has obtained a license from the Ethics Committee on Animal Use of the School of Veterinary Medicine and Zootechnics of the Federal University of Bahia, with authorization protocol number 0733770120200504, and is registered in the SISGEN database under number AB07806. A total of 22 samples were collected from oropharynx (n=14), cloaca (n=7), and ocular secretion (n=1) of 19 adult specimens of Amazona aestiva, using sterile swabs. The samples were cultured on Sabouraud Dextrose agar with chloramphenicol, with and without lipid supplementation, and incubated in a bacteriological incubator at 32 °C for 5 days. Molecular identification of the yeasts was performed by Sanger sequencing of the ITS1-5.8S-ITS2 region using the ITS1 and ITS4 primers. The sequences were compared using BLAST in the BoldSystems and GenBank databases. Identification was considered acceptable when a match was obtained with an E-value of 0.0, a minimum coverage of 98%, and an identity of 99%.

Resultados e Conclusão

All samples showed growth of at least one yeast. A total of 23 yeasts were isolated, belonging to the species Debaryomyces hansenii (n=10), Candida parapsilosis (n=6), Lodderomyces elongisporus (n=6), and Candida orthopsilosis (n=1). D. hansenii, C. parapsilosis, and C. orthopsilosis are emerging yeasts in humans, reported in the last decade as agents of fungemia. The results of this study highlight the potential of wild birds as possible disseminators of emerging and opportunistic yeasts.

Palavras-chave

zoonosis; wildlife

Agradecimentos

FAPESB, SASE e CETAB.