Dados do Trabalho

Título

SELECTION AND CHARACTERIZATION OF APPLICABLE BIOMARKERS IN NANOTECHNOLOGICAL PLATFORMS FOR THE DIAGNOSIS OF TUBERCULOSIS

Introdução

Tuberculosis (TB) is an infectious disease with airborne dissemination caused by Mycobacterium tuberculosis that primarily affects the lungs, although it can affect other organs and systems depending on the degree of immunosuppression of the patient. Despite a long documented history of reports of tuberculosis, the disease remains a global public health problem, under which the World Health Organization mobilizes efforts to reduce new cases of the disease and the number of deaths, avoiding suffering caused by the disease. Several limitations in currently used bacteriological and radiological tests indicate that an improvement in the detection of new cases of TB using noninvasive samples is critical to contain the spread of this neglected disease.

Objetivo (s)

This study aimed to develop peptides that mimic regions of antigenic proteins of M. tuberculosis that can be used in salivary diagnosis.

Material e Métodos

Phage display technology was used to randomly select peptides against specific IgA of M. tuberculosis using saliva samples from tuberculosis treatment-naive (TB) patients with positive bacterioscopy/culture and radiological findings. After selection, individual phage clones were randomly chosen and amplified. Single-stranded DNA was extracted and sequenced, and the reactivity was evaluated by enzyme-linked immunoassay (ELISA).

Resultados e Conclusão

Sixteen phage clones were identified after DNA sequencing, and 9 were selected based on three-dimensional alignment with M. tuberculosis proteins using a bioinformatics approach. Then, the most reactive peptide in ELISA was synthesized TBC10 and duplicated TB2C10. A highly reactive phage C10 and a synthetic duplicated peptide TB2C10 against specific IgA of M. tuberculosis were capable of discriminating TB patients from subjects with a negative diagnosis for TB with a specificity of 100% and a sensitivity of 76.9% for phage and specificity of 92.3% and a sensitivity of 88.2% for peptide TB2C10. These findings suggest that a TB detection assay using peptides may offer a new noninvasive alternative platform for future use in point-of-care salivary diagnosis.

Palavras-chave

Tuberculosis, saliva, Mycobacterium tuberculosis, Phage display, peptides, salivary diagnostics.

Agradecimentos

This research was funded by National Institute of Science and Technology in Theranostics and Nanobiotechnology – INCT-Teranano (CNPq/CAPES/FAPEMIG, CNPq-403193/2022-2, FAPEMIG APQ-03613-17).