Dados do Trabalho

Título

Immunoglobulin Y for potential diagnostic and treatment of SARS-CoV-2

Introdução

SARS-CoV-2 have high transmission rate and could cause since asymptomatic infections to fatal disease and long-Covid. Until this moment, SARS-CoV-2 is responsible for more than 760 million cases and 6,9 million deaths globally which turns extremely important the use of new immunodiagnostic assays and treatments. One alternative is the Immunoglobulin Y (IgY), present in egg yolk and serum of birds. These antibodies have certain advantages when compared to mammals IgG: less production expense, high efficiency, increased humoral immune response against mammalian proteins, reduction in background colour of immunoenzymatic assays. Besides that, the extraction is simpler than IgG and can be made merely by egg yolk collection instead of animals bleeding. These advantages turn IgY an alternative to immunodiagnostic and treatment of viral infections such as SARS-CoV-2. In this way, this work has the aim to product IgY anti-SARS-CoV in immunized chicken and evaluate this antibody to the development of new immunodiagnostic assays and treatment of SARS-CoV-2.

Objetivo (s)

This work has the aim to product IgY anti-SARS-CoV in immunized chicken and evaluate this antibody to the development of new immunodiagnostic assays and treatment of SARS-CoV-2.

Material e Métodos

Isa brown chickens (Gallus gallus domesticus) were separated in 2 groups: (1) Immunogenic group (with 2 chickens) inoculated with inactivated SARS-CoV-2 virus, recombinant SARS-CoV-2 Spike protein and with the adjuvants IFA and CpG-ODN; (2) Negative control group (with one chicken) was inoculated with the adjuvants IFA and CpG-ODN. All animals were inoculated 5 times. After 20 weeks the eggs were collected daily and the IgY was purified from egg yolk and characterized by SDS-PAGE. Besides that, IgY efficacy against SARS-CoV-2 was evaluated by the determination of neutralizing antibodies.

Resultados e Conclusão

The immunized animals do not show different weight loss and egg-laying performance than the negative control. SDS-PAGE profile demonstrates the presence of IgY in egg yolk from the immunized chicken. IgY concentration was measured by spectrophotometry and was of 3.4 mg/ml. One chicken from the immunogenic group showed high antibody neutralization rate (96.89%) at 17 week after the first immunization. In conclusion, the IgY anti-SARS-CoV-2 produced in chickens showed high concentration, high antibody neutralization rate and could be used to design new immunodiagnostic assay and immunotherapy.

Palavras-chave

SARS-CoV-2; IgY; Treatment; Imumunoassays

Agradecimentos

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