Dados do Trabalho

Título

Trypanosoma diversity of free-living mammals in urban fragments in Central-Western Brazil

Introdução

Trypanosoma spp. are ubiquitous protozoans that are maintained in the environment through complex cycles parasitizing humans, domestic mammals, and wildlife, but prevalence and maintenance in wild hosts found in urban fragments of the city are still unknown.

Objetivo (s)

So we aimed to access the Trypanosoma diversity in mammals that live in forest fragments of Campo Grande municipality, Mato Grosso do Sul, Brazil.

Material e Métodos

From May 2017 to November 2020, we sampled in Campo Grande, Mato Grosso do Sul, 457 individuals belonging to 18 species of free-living bats, small mammals, coatis, and capibaras, that live in forest fragments. Mammals were captured, sedated, and biological samples were collected according to the recommended methodologies for each group. Hemoculture was performed to determine patent infections (high parasitemias), molecular assay was made in order to detect cryptic infections, and serology was used to indicate exposition.

Resultados e Conclusão

We found that Trypanosoma cruzi DTU I and T. cruzi DTU II circulate among opossums (Didelphis albiventris) and primates (Sapajus cay and Alouatta caraya) in cryptic parasitemias, while in bats (Artibeus lituratus and Eptesicus. furinalis) we observed high parasitemias of T. cruzi DTU I. Moreover, we detect T. cruzi DTU IV in A. caraya and S. cay. We also observed high parasitemias by T. cruzi marinkelei in Artibeus planirostris, Trypanosoma dionisii in E. furinalis and Sturnira lilium, and Trypanosoma rangeli in S. cay. For the first time recorded high parasitemias by Trypanosoma janseni in S. lilium, as well as Trypanosoma terrestris in Hydrochaerus hydrochaeris. Furthermore, we detected Trypanosoma lainsoni in D. albiventris and S. cay, Trypanosoma minasense in A. caraya and S. cay, and Trypanosoma sp. DID in D. albiventris, A. caraya, and S. cay. The high diversity of Trypanosoma observed in Campo Grande could be a consequence of the presence of many wild mammalian host species. The presence of new records such as T. janseni and T. terrestris in an urban area highlighted that our knowledge of the transmission cycles of Trypanosoma are still incipient and should be better explored.

Palavras-chave

Trypanosomatids; Urban Fauna, Wildlife

Agradecimentos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); FAPERJ.